The dynamics of nitric oxide release measured directly and in real time following repeated waves of cortical spreading depression in the anaesthetised cat

Neurosci Lett. 1997 Sep 5;232(3):127-30. doi: 10.1016/s0304-3940(97)00604-6.


Cortical application of crystalline KCl in male cats anaesthetised with alpha-chloralose induced four transient negative deflections in cortical direct current (d.c.) potential. In vehicle treated animals d.c. shifts were associated with a hyperaemia and a multiphasic nitric oxide (NO) release. In these animals, the first negative shift in d.c. potential produced a significantly larger NO electrode current, when compared to subsequent cortical depolarisations. However, regional cerebrovascular laser Doppler flux (rCBF[LDF]) increases were similar for each event. In L-N(G)-nitro-L-arginine (L-NAME; 10 mg/kg i.v. infused over 30 min) treated animals, d.c. shifts were also associated with NO release following a multiphasic waveform and increase in rCBF(LDF), but were significantly attenuated when compared to controls.

MeSH terms

  • Anesthesia
  • Animals
  • Cats
  • Cortical Spreading Depression / drug effects
  • Cortical Spreading Depression / physiology*
  • Enzyme Inhibitors / pharmacology
  • Male
  • NG-Nitroarginine Methyl Ester / pharmacology
  • Nitric Oxide / metabolism*
  • Time Factors


  • Enzyme Inhibitors
  • Nitric Oxide
  • NG-Nitroarginine Methyl Ester