Glycerol conversion to 1,3-propanediol by Clostridium pasteurianum: cloning and expression of the gene encoding 1,3-propanediol dehydrogenase

FEMS Microbiol Lett. 1997 Sep 15;154(2):337-45. doi: 10.1111/j.1574-6968.1997.tb12665.x.


When grown on glycerol as sole carbon and energy source, cell extracts of Clostridium pasteurianum exhibited activities of glycerol dehydrogenase, dihydroxyacetone kinase, glycerol dehydratase and 1,3-propanediol dehydrogenase. The genes encoding the latter two enzymes were cloned by colony hybridization using the dhaT gene of Citrobacter freundii as a heterologous DNA probe and expressed in Escherichia coli. The native molecular mass of 1,3-propanediol dehydrogenase (DhaT) is 440,000 Da. The dhaT gene of C. pasteurianum was subcloned and its nucleotide sequence (1158 bp) was determined. The deduced gene product (41,776 Da) revealed high similarity to DhaT of C. freundii (80.5% identity; 89.8% similarity).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Dehydrogenase
  • Alcohol Oxidoreductases / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Clostridium / genetics
  • Clostridium / metabolism*
  • Genes, Bacterial*
  • Glycerol / metabolism*
  • Molecular Sequence Data
  • Propylene Glycols / metabolism*


  • Propylene Glycols
  • 1,3-propanediol
  • Alcohol Oxidoreductases
  • Alcohol Dehydrogenase
  • 1,3-propanediol dehydrogenase
  • Glycerol

Associated data

  • GENBANK/AF006034