[Topography of fundus autofluorescence with a new confocal scanning laser ophthalmoscope]

Ophthalmologe. 1997 Jun;94(6):385-91. doi: 10.1007/s003470050130.
[Article in German]


Background: We investigated fundus autofluorescence in vivo using a novel scanning laser ophthalmoscope.

Materials and methods: A total of 550 patients with various retinal diseases were examined and compared with normal eyes. Autofluorescence was detected after excitation with an argon blue laser (488 nm), and emission was recorded with a short wavelength cut off above 500 nm.

Results: Reduced autofluorescence was observed in the foveal and parafoveal region due to retinal xanthophyll, along the retinal vessels, at the optic nerve head and in areas with atrophy of the retinal pigment epithelium (RPE). Autofluorescence intensity was increased either focally or diffusely in certain degenerative (AMD) or genetically determined retinal diseases (e.g., Stargardt's disease, Best's disease).

Conclusions: These findings are in accordance with the view that in vivo fundus autofluorescence originates at the level of the RPE and suggest that it is derived from lipofuscin.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Female
  • Fundus Oculi*
  • Humans
  • Image Processing, Computer-Assisted / instrumentation
  • Lipofuscin / metabolism
  • Lutein / metabolism
  • Male
  • Microscopy, Confocal / instrumentation*
  • Microscopy, Fluorescence / instrumentation*
  • Middle Aged
  • Ophthalmoscopes*
  • Optic Disk / pathology
  • Pigment Epithelium of Eye / pathology
  • Retina / pathology
  • Retinal Diseases / diagnosis*
  • Retinal Diseases / genetics


  • Lipofuscin
  • Lutein