Effects of exposure to electromagnetic radiation at 835 MHz on growth, morphology and secretory characteristics of a mast cell analogue, RBL-2H3

Cell Biol Int. 1997 Jul;21(7):427-39. doi: 10.1006/cbir.1997.0162.


A mast cell line, RBL-2H3, was exposed to 835 MHz for 20 minutes, three times per day for 7 days at a power density of 8.1 +/- 3 mW/cm2. From day 4 onwards, it was observed that the rate of DNA synthesis and cell replication increased, that actin distribution and cell morphology became altered, and the amount of beta-hexosaminidase (a marker of granule secretion) released in response to a calcium ionophore was significantly enhanced, in comparison to unexposed cultures. There were no effects seen on levels of cytoskeletal protein synthesis or of beta-actin mRNA. Morphological changes persisted following subculture for at least 7 days in the absence of further exposure. It is hypothesized that effects of exposure to an electromagnetic field at 835 MHz may be mediated via a signal transduction pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / analysis
  • Animals
  • Cell Division / radiation effects
  • Cell Line
  • Cell Size / radiation effects
  • DNA / biosynthesis
  • Mast Cells / cytology
  • Mast Cells / radiation effects*
  • Radiation*
  • Rats
  • beta-N-Acetylhexosaminidases / metabolism


  • Actins
  • DNA
  • beta-N-Acetylhexosaminidases