Molecular cloning of rat p38 mitogen-activated protein kinase and it's osmotic regulation in rat kidney

Biochem Mol Biol Int. 1997 Sep;43(1):63-72. doi: 10.1080/15216549700203821.


Rat p38 mitogen-activated protein (MAP) kinase cDNA was isolated from rat kidney cDNA library using a PCR cloning strategy. The deduced amino acid sequence consists of 360 amino acids and shares 95.3% similarity with human p38 MAP kinase. The message for rat p38 MAP kinase was about 3.4 kilobases and was highly expressed in the kidney. In water-deprived rat kidneys, the steady-state levels of p38 MAP kinase mRNA increased about 2.7-fold as compared with those of control rats. This result suggests that p38 MAP kinase may play an important role in the osmoregulation in the kidney.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calcium-Calmodulin-Dependent Protein Kinases / chemistry
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics*
  • Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
  • Cloning, Molecular
  • DNA, Complementary
  • Humans
  • Kidney / enzymology*
  • Male
  • Mitogen-Activated Protein Kinases*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Wistar
  • Sequence Alignment
  • Water-Electrolyte Balance*
  • p38 Mitogen-Activated Protein Kinases


  • DNA, Complementary
  • RNA, Messenger
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Mitogen-Activated Protein Kinases
  • p38 Mitogen-Activated Protein Kinases

Associated data

  • GENBANK/AB000783