Astrocyte-conditioned medium induces blood-brain barrier properties in endothelial cells

Clin Exp Pharmacol Physiol. 1997 Sep-Oct;24(9-10):710-3. doi: 10.1111/j.1440-1681.1997.tb02117.x.


1. We evaluated the induction of endothelial barrier functions by the type 1 astrocyte-derived supernatant in culture using horseradish peroxidase (HRP) as a parameter. 2. A gel filtration fraction (molecular weight 16-20 kDa) of type 1 astrocyte-derived supernatant reduced plasmalemmal vesicles and induced tight junctions in endothelial cells. 3. In addition, this fraction promoted the uptake of [3H]-glucosamine by endothelial cells. This cell activity disappeared after heating at 95 degrees C for 10 min and was sensitive to trypsin. 4. These results suggest that a type 1 astrocyte-derived protein factor(s) promotes proteoglycan synthesis with a resultant increase in charge selectivity in endothelial cells and plays an important role in the induction of blood-brain barrier functions.

MeSH terms

  • Animals
  • Astrocytes / drug effects
  • Astrocytes / metabolism
  • Astrocytes / physiology*
  • Blood-Brain Barrier / drug effects
  • Blood-Brain Barrier / physiology*
  • Cells, Cultured
  • Chromatography, Gel
  • Culture Media, Conditioned / pharmacology*
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / physiology*
  • Glucosamine / metabolism
  • Horseradish Peroxidase
  • Microscopy, Electron
  • Molecular Weight
  • Rats


  • Culture Media, Conditioned
  • Horseradish Peroxidase
  • Glucosamine