The immediate-early gene product MAD-3/EDG-3/IkappaB alpha is an endogenous modulator of fibroblast growth factor-1 (FGF-1) dependent human endothelial cell growth

FEBS Lett. 1997 Sep 8;414(2):419-24. doi: 10.1016/s0014-5793(97)01053-3.


The tumor promoter phorbol 12-myristic 13-acetate inhibits the growth of human endothelial cells and induces the formation of capillary-like, tubular structures. We report the novel growth regulatory function of the immediate-early gene, edg-3, which is identical to the IkappaB alpha/MAD-3 gene. We employed phosphothioate oligonucleotides (PTO) directed against the translation initiation site of IkappaB alpha to inhibit its expression. The antisense IkappaB alpha PTO-treated cells exhibited an exaggerated growth response to fibroblast growth factor-1 (FGF-1). In contrast, IL-1-induced growth arrest response was not modulated. These data suggest that the early response gene IkappaB alpha is an endogenous regulator of endothelial cell growth in vitro.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Division / drug effects
  • Cells, Cultured
  • Cycloheximide / pharmacology
  • DNA-Binding Proteins / biosynthesis*
  • Endothelium, Vascular / cytology*
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism*
  • Fibroblast Growth Factor 1 / pharmacology*
  • Genes, Immediate-Early / drug effects
  • Humans
  • I-kappa B Proteins*
  • Immediate-Early Proteins / biosynthesis*
  • NF-KappaB Inhibitor alpha
  • Oligonucleotides, Antisense / pharmacology
  • Polymerase Chain Reaction
  • Protein Biosynthesis
  • Receptors, Lysophospholipid
  • Tetradecanoylphorbol Acetate / pharmacology
  • Thionucleotides
  • Umbilical Veins


  • DNA-Binding Proteins
  • I-kappa B Proteins
  • Immediate-Early Proteins
  • NFKBIA protein, human
  • Oligonucleotides, Antisense
  • Receptors, Lysophospholipid
  • Thionucleotides
  • Fibroblast Growth Factor 1
  • NF-KappaB Inhibitor alpha
  • Cycloheximide
  • Tetradecanoylphorbol Acetate