PCR-enzyme-linked immunosorbent assay and sequencing as an alternative to serology for M-antigen typing of Streptococcus pyogenes

J Clin Microbiol. 1997 Oct;35(10):2689-91. doi: 10.1128/jcm.35.10.2689-2691.1997.

Abstract

A rapid PCR-enzyme-linked immunosorbent assay for identification of 10 important emm gene types of Streptococcus pyogenes was developed. The emm genotypes of a coded panel of strains of known M serotype were determined, and in 144 of 149 cases (97%) the results were congruous. Strains of types that were not included in the panel of capture probes were emm genotyped by sequencing.

Publication types

  • Comparative Study

MeSH terms

  • Antigens, Bacterial*
  • Bacterial Outer Membrane Proteins*
  • Bacterial Proteins / genetics*
  • Biological Specimen Banks
  • Carrier Proteins*
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Genotype
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods*
  • Reference Standards
  • Sequence Analysis, DNA*
  • Serotyping
  • Streptococcus pyogenes / classification*

Substances

  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Carrier Proteins
  • streptococcal M protein

Associated data

  • GENBANK/M20374