Abstract
Prostatic acid phosphatase (PAP) is uniquely expressed in prostatic tissue and prostate cancer. In this study, the immunogenicity of PAP was investigated in a male rat model. We show that immunization with recombinant rat or human PAP in CFA leads to a significant Ab response, but does not generate CTL or result in autoimmune prostatitis. In contrast, immunization with recombinant vaccinia expressing human PAP, but not rat PAP, generates a CTL response and tissue-specific prostatitis in the absence of detectable PAP-specific Abs. These findings suggest that a cellular immune response to PAP, rather than Abs, mediates destructive autoimmune prostatitis. Thus, xenogeneic forms of PAP are a new tool for the induction of prostate-specific immunity and may prove useful for the immunotherapy of prostate cancer.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acid Phosphatase / administration & dosage
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Acid Phosphatase / genetics
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Acid Phosphatase / immunology*
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Animals
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Autoimmune Diseases / enzymology
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Autoimmune Diseases / etiology*
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Autoimmune Diseases / therapy*
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Humans
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Immunotherapy, Active
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Injections, Intravenous
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Injections, Subcutaneous
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Male
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Organ Specificity / immunology
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Prostate / enzymology*
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Prostatic Neoplasms / enzymology
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Prostatic Neoplasms / immunology*
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Prostatic Neoplasms / therapy*
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Prostatitis / enzymology
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Prostatitis / etiology*
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Prostatitis / immunology*
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Rats
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Rats, Inbred Strains
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Tumor Cells, Cultured
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Vaccines, Synthetic / administration & dosage
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Vaccines, Synthetic / immunology
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Vaccinia virus / genetics
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Vaccinia virus / immunology
Substances
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Vaccines, Synthetic
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Acid Phosphatase