Initiation and bidirectional propagation of chromatin assembly from a target site for nucleotide excision repair

EMBO J. 1997 Oct 15;16(20):6281-9. doi: 10.1093/emboj/16.20.6281.


To restore full genomic integrity in a eukaryotic cell, DNA repair processes have to be coordinated with the resetting of nucleosomal organization. We have established a cell-free system using Drosophila embryo extracts to investigate the mechanism linking de novo nucleosome formation to nucleotide excision repair (NER). Closed-circular DNA containing a uniquely placed cisplatin-DNA adduct was used to follow chromatin assembly specifically from a site of NER. Nucleosome formation was initiated from a target site for NER. The assembly of nucleosomes propagated bidirectionally, creating a regular nucleosomal array extending beyond the initiation site. Furthermore, this chromatin assembly was still effective when the repair synthesis step in the NER process was inhibited.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell-Free System
  • Chromatin / metabolism*
  • Cisplatin / metabolism
  • DNA / biosynthesis
  • DNA Adducts / metabolism
  • DNA Repair*
  • DNA, Circular / metabolism
  • Drosophila
  • Embryo, Nonmammalian
  • Humans
  • Models, Genetic
  • Xenopus


  • Chromatin
  • DNA Adducts
  • DNA, Circular
  • DNA
  • Cisplatin