Construction of specific DNA probe for the detection of Salmonella in food

A Pilantanapak; P Jayanetra; W Panbangred; C Klungthong; A Bangtrakulnonth

Construction of specific DNA probe for the detection of Salmonella in food

Southeast Asian J Trop Med Public Health. 1997 Mar;28(1):73-81.

Authors

A Pilantanapak¹, P Jayanetra, W Panbangred, C Klungthong, A Bangtrakulnonth

Affiliation

¹ Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

PMID: 9322287

Abstract

The Salmonella specific DNA fragment from genomic DNA of S. typhimurium ATCC 23566 was cloned in E. coli and successfully used as a digoxigenin labeled probe for detecting the presence of Salmonella serotypes in both artificially contaminated food and natural contaminated food samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cloning, Molecular
Colony Count, Microbial
DNA Probes / genetics*
DNA, Bacterial / genetics
Digoxigenin
Escherichia coli / genetics
Food Microbiology*
Humans
Immunoblotting
Salmonella Food Poisoning / microbiology*
Salmonella Food Poisoning / prevention & control
Salmonella typhimurium / genetics*

Substances

DNA Probes
DNA, Bacterial
Digoxigenin

Associated data

GENBANK/AF025748