Identification of N-WASP homologs in human and rat brain

Gene. 1997 Sep 1;196(1-2):43-8. doi: 10.1016/s0378-1119(97)00184-4.


We recently identified Neural Wiskott-Aldrich Syndrome Protein (N-WASP) from bovine brain. An expression analysis using bovine cDNA revealed that N-WASP plays critical roles in the regulation of the cortical actin cytoskeleton. Here, we report the molecular cloning of N-WASP homologs from human and rat brain cDNA libraries. The predicted amino acid sequences of human and rat N-WASP show 96% and 95% identity to bovine N-WASP, respectively, suggesting the functional importance of the molecule. Antibody raised against recombinant rat N-WASP recognizes a 65-kDa protein that exists ubiquitously in whole brain, including cerebrum, cerebellum, interbrain, and medulla oblongata. N-WASP was shown to be concentrated at the nerve terminal region. The gene locus of human N-WASP was also determined at 7q31.3 by fluorescence in-situ hybridization (FISH) analysis.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Western
  • Brain / metabolism*
  • Cattle
  • Chromosome Mapping
  • Chromosomes, Human, Pair 7
  • Cloning, Molecular
  • Conserved Sequence
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / immunology
  • Nerve Tissue Proteins / metabolism*
  • Rats
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Wiskott-Aldrich Syndrome Protein, Neuronal


  • Nerve Tissue Proteins
  • Recombinant Proteins
  • WASL protein, human
  • Wasl protein, rat
  • Wiskott-Aldrich Syndrome Protein, Neuronal

Associated data

  • GENBANK/D88460
  • GENBANK/D88461