Alpha-tocopherol does not inhibit hypochlorite-induced oxidation of apolipoprotein B-100 of low-density lipoprotein

FEBS Lett. 1997 Sep 15;414(3):541-4. doi: 10.1016/s0014-5793(97)01066-1.

Abstract

The amount of alpha-tocopherol (alpha-TOH) can dramatically alter the extent of radical-induced oxidation of low density lipoprotein (LDL) lipids, a process generally thought to be important in atherogenesis. However, LDL with atherogenic features can also be formed in vitro by exposure to the strong non-radical oxidant hypochlorite (HOCl), which preferentially oxidises LDL apolipoprotein B-100. Here we show that varying LDL content of alpha-TOH by vitamin supplementation or depletion has no effect on the extent of HOCI-induced oxidation of apolipoprotein B-100 as measured by the loss of lysine and tryptophan residues, and the alteration in relative electrophoretic mobility of the lipoprotein particle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Apolipoprotein B-100
  • Apolipoproteins B / drug effects
  • Apolipoproteins B / metabolism*
  • Female
  • Humans
  • Hypochlorous Acid / pharmacology*
  • Lipoproteins, LDL / chemistry
  • Lipoproteins, LDL / drug effects
  • Lipoproteins, LDL / metabolism
  • Lysine / metabolism
  • Male
  • Oxidation-Reduction
  • Tryptophan / metabolism
  • Vitamin E / chemistry
  • Vitamin E / pharmacology*

Substances

  • Apolipoprotein B-100
  • Apolipoproteins B
  • Lipoproteins, LDL
  • Vitamin E
  • Hypochlorous Acid
  • Tryptophan
  • Lysine