Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjögren's syndrome

Arthritis Rheum. 1997 Sep;40(9):1693-702. doi: 10.1002/art.1780400920.


Objective: To identify a Golgi complex autoantigen bound by Sjögren's syndrome (SS) autoantibodies.

Methods: Serum from a patient with secondary SS and anti-Golgi antibodies was used as a probe to isolate a complementary DNA (cDNA) insert from a HeLa cDNA library.

Results: A 3.7-kb cDNA encoding a 56-kd recombinant protein was immunoprecipitated by the human anti-Golgi serum and immune rabbit serum. Western blot analysis showed that the immune rabbit sera recognized a protein of 97 kd (golgin-97), suggesting that the isolated clone contained a partial cDNA. The 5' upstream sequence was obtained by rapid amplification of the cDNA ends. The complete cDNA contained 4,860 basepairs, encoding a protein with a calculated Mr of 88 kd. Antibodies to golgin-97 were found in 12 (20%) of 60 sera known to have anti-Golgi autoantibodies, and the majority of these sera (8 of 12, or 75%) were from patients who had secondary SS.

Conclusion: Golgin-97 is a unique Golgi complex antigen that appears to be a target of SS autoantibodies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Autoantibodies / analysis
  • Autoantigens / genetics*
  • Autoantigens / immunology
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA Primers / chemistry
  • DNA, Complementary / isolation & purification
  • Female
  • Golgi Apparatus / immunology*
  • Golgi Matrix Proteins
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Sjogren's Syndrome / immunology*


  • Autoantibodies
  • Autoantigens
  • DNA Primers
  • DNA, Complementary
  • Golgi Matrix Proteins
  • Golgi complex autoantigen, 97-kDa

Associated data

  • GENBANK/U51587