Changes in the V(H) gene repertoire of developing precursor B lymphocytes in mouse bone marrow mediated by the pre-B cell receptor

Immunity. 1997 Sep;7(3):357-68. doi: 10.1016/s1074-7613(00)80357-x.


The V(H) repertoire on both H chain alleles of normal and lambda5-deficient B lineage cells were analyzed by single-cell PCR. The mu H chains were tested for their capacity to form a pre-B cell receptor. In bone marrow, D-proximal V(H) genes were found preferentially expressed in lambda5-deficient pre-B cells and in a newly identified early c-kit+ cytoplasmic mu H chain+ pre-B cell population of normal mice. Only half of the mu H chains expressed in these cells have the capacity to form a pre-B cell receptor. Representation of the D-proximal V(H) genes was found suppressed on the productive but not on the nonproductive V(H)DJ(H) rearranged alleles of c-kit preB-II cells and splenic lambda5-deficient B cells. More than 95% of the mu H chains expressed in preB-II cells can form a pre-B cell receptor. These results demonstrate that the pre-B cell receptor in normal mice and the B cell receptor in lambda5-deficient mice mediate a shift in the V(H) repertoire.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Animals
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / physiology
  • B-Lymphocytes / ultrastructure
  • Base Sequence
  • Bone Marrow / physiology
  • Bone Marrow / ultrastructure
  • Bone Marrow Cells*
  • Female
  • Genes, Immunoglobulin*
  • Immunoglobulin Variable Region / genetics*
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Protein Precursors / genetics
  • Proto-Oncogene Proteins c-kit / metabolism
  • Receptors, Antigen, B-Cell / physiology*


  • Immunoglobulin Variable Region
  • Protein Precursors
  • Receptors, Antigen, B-Cell
  • Proto-Oncogene Proteins c-kit