cDNA cloning of a short type of multidrug resistance protein homologue, SMRP, from a human lung cancer cell line

Biochem Biophys Res Commun. 1997 Sep 29;238(3):790-4. doi: 10.1006/bbrc.1997.7346.


Members of the ATP binding cassette (ABC) superfamily are involved in the energy-dependent transport of a wide variety of substrates including anticancer agents across the membranes. We have cloned a cDNA fragment including a novel ABC sequence from a cisplatin-resistant human lung adenocarcinoma cell line, PC-14/CDDP, by reverse transcription polymerase chain reaction (RT-PCR) using degenerate primers and screened a cDNA library using the cDNA fragment as a probe. A full-length cDNA clone, BM4.8, was obtained. Sequence analysis showed that the cDNA encoded a short type of multidrug resistance protein homologue, SMRP, by computed homology search. SMRP was composed of 946 amino acids and had two ABCs with walker A and B motifs. This gene was mapped on chromosome 3 at band q27 by fluorescence in situ hybridization (FISH) analysis and was found to be expressed in various tissues by Northern blot analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • ATP-Binding Cassette Transporters / isolation & purification
  • Amino Acid Sequence
  • Base Sequence
  • Carcinoma, Non-Small-Cell Lung / chemistry
  • Carcinoma, Non-Small-Cell Lung / genetics*
  • Chromosome Mapping
  • Chromosomes, Human, Pair 3
  • Cisplatin / pharmacology
  • Cloning, Molecular
  • DNA, Complementary / isolation & purification*
  • Drug Resistance, Multiple*
  • Humans
  • Lung Neoplasms / chemistry
  • Lung Neoplasms / genetics*
  • Molecular Sequence Data
  • Multidrug Resistance-Associated Proteins
  • Multigene Family
  • Neoplasm Proteins / genetics*
  • Neoplasm Proteins / isolation & purification
  • Polymerase Chain Reaction
  • Tumor Cells, Cultured


  • ATP-Binding Cassette Transporters
  • DNA, Complementary
  • Multidrug Resistance-Associated Proteins
  • Neoplasm Proteins
  • Cisplatin

Associated data

  • GENBANK/AB005659