Anti-tumor activity of squid ink

J Nutr Sci Vitaminol (Tokyo). 1997 Aug;43(4):455-61. doi: 10.3177/jnsv.43.455.


The anti-tumor activity of a new type of peptidoglycan isolated from squid ink was shown to have a cure rate of 64% for Meth A tumor from BALB/c mice. The ink delipidated in acetone, which contained the peptidoglycan at 0.1% (w/w), was administered to tumor-transplanted mice so as to examine the anti-tumor activity. One-fifth of the tumor-bearing mice was cured with 3 injections (1 mg/head) of the acetone delipidated squid ink or a prolongation of survival was observed in the treated animals. Heat treatment at 100 degrees C for 10 min did not affect the anti-tumor activity of the delipidated ink, its potentiality being preserved. The acetone-extractable fraction of the ink also brought about a similar cure rate for Meth A tumor. The delipidated ink enhanced the phagocytic activity of macrophages but no direct cytotoxicity was observed for the Meth A tumor cells. Hence it may be said that the anti-tumor activity of the delipidated ink was mainly due to the augmented cellular immunity in vivo.

MeSH terms

  • Acetone
  • Acetylgalactosamine / analysis
  • Animals
  • Antineoplastic Agents / therapeutic use*
  • Carcinogens
  • Decapodiformes / metabolism*
  • Drug Stability
  • Fibrosarcoma / chemically induced
  • Fibrosarcoma / drug therapy*
  • Fucose / analysis
  • Glucuronates / analysis
  • Glucuronic Acid
  • Hot Temperature
  • Lipids
  • Melanins / analysis
  • Mice
  • Mice, Inbred BALB C
  • Neoplasm Transplantation
  • Peptidoglycan / analysis
  • Peptidoglycan / isolation & purification
  • Peptidoglycan / therapeutic use*
  • Polysaccharides / analysis


  • Antineoplastic Agents
  • Carcinogens
  • Glucuronates
  • Lipids
  • Melanins
  • Peptidoglycan
  • Polysaccharides
  • Acetone
  • Fucose
  • Glucuronic Acid
  • Acetylgalactosamine