Cell cycle-dependent protein expression of mammalian homologs of yeast DNA double-strand break repair genes Rad51 and Rad52

Mutat Res. 1997 Sep;384(3):205-11. doi: 10.1016/s0921-8777(97)00020-7.


Recently, human and rodent homologs of yeast repair genes Rad51 and Rad52 have been identified and proposed to play roles in DNA double-strand break (DSB) repair. In this study, cell cycle-dependent expression of human and rodent RAD51 and RAD52 proteins was monitored using two approaches. First, flow cytometric measurements of DNA content and immunofluorescence were used to determine the phase-specific levels of RAD51 and RAD52 protein expression in irradiated and control populations. The expression of both proteins was lowest in G0/G1, increased in S and reached a maximum in G2/M. No difference was found in the whole-cell level of RAD51 or RAD52 protein expression between gamma-irradiated and control cell populations. Second, cell cycle-dependent protein expression was confirmed by Western analysis of populations synchronized in G0, G1 and G2 phases. Analysis of V3, a hamster equivalent of SCID, indicates that the protein level increases of RAD51 and RAD52 from G0 to G1/S/G2 do not require DNA-PK.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CHO Cells
  • Cell Cycle / genetics*
  • Cell Cycle / radiation effects
  • Cells, Cultured
  • Cesium Radioisotopes
  • Cricetinae
  • DNA Repair* / radiation effects
  • DNA-Binding Proteins / biosynthesis*
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / radiation effects
  • Fibroblasts
  • Fungal Proteins / biosynthesis*
  • Fungal Proteins / genetics*
  • Fungal Proteins / radiation effects
  • Gene Expression Regulation / radiation effects
  • Humans
  • Molecular Sequence Data
  • Rad51 Recombinase
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins
  • Sequence Homology, Amino Acid
  • Skin


  • Cesium Radioisotopes
  • DNA-Binding Proteins
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • RAD51 protein, S cerevisiae
  • RAD51 protein, human
  • Rad51 Recombinase