Expression of neural RNA-binding proteins in the postnatal CNS: implications of their roles in neuronal and glial cell development

J Neurosci. 1997 Nov 1;17(21):8300-12. doi: 10.1523/JNEUROSCI.17-21-08300.1997.


There is an increasing interest in the role of RNA-binding proteins during neural development. Mouse-Musashi-1 (m-Msi-1) is a mouse neural RNA-binding protein with sequence similarity to Drosophila musashi (d-msi), which is essential for neural development. m-Msi-1 is highly enriched in neural precursor cells that are capable of generating both neurons and glia during embryonic CNS development. The present study characterized m-Msi-1-expressing cells in the postnatal and adult CNS. Postnatally, m-Msi-1 was expressed in proliferative neuronal precursors in the external granule cell layer of the cerebellum and in the anterior corner of the subventricular zone of the lateral ventricles. In gliogenesis, the persistent expression of m-Msi-1 was observed in cells of the astrocyte lineage ranging from proliferative glial precursors in the subventricular zone (SVZ) to differentiated astrocytes in the parenchyma. In addition, we showed that m-Msi-1 was still expressed in proliferating cells in the adult SVZ, which may contain neural precursor or stem cells. Another neural RNA-binding protein Hu (the mammalian homolog of a Drosophila neuronal RNA-binding protein Elav) was present in postmitotic neurons throughout the development of the CNS, and its pattern of expression was compared with that of m-Msi-1. These observations imply that these two RNA-binding proteins may be involved in the development of neurons and glia by regulating gene expression at the post-transcriptional level.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes / cytology
  • Astrocytes / metabolism*
  • Brain / cytology
  • Brain / embryology
  • Brain / growth & development
  • Brain / metabolism*
  • Cell Differentiation
  • Cell Lineage
  • Cerebral Cortex / cytology
  • Cerebral Cortex / embryology
  • Cerebral Cortex / growth & development
  • Cerebral Cortex / metabolism
  • Gene Expression Regulation, Developmental*
  • Mice
  • Mice, Inbred ICR
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / physiology
  • Neurons / cytology*
  • Neurons / metabolism
  • Organ Specificity
  • Prosencephalon / cytology
  • Prosencephalon / embryology
  • Prosencephalon / growth & development
  • Prosencephalon / metabolism
  • RNA-Binding Proteins / biosynthesis*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / physiology
  • Rats
  • Rats, Wistar
  • Stem Cells / cytology
  • Stem Cells / metabolism


  • Msi1 protein, rat
  • Msi1h protein, mouse
  • Nerve Tissue Proteins
  • RNA-Binding Proteins