The AP-3 Adaptor Complex Is Essential for Cargo-Selective Transport to the Yeast Vacuole

Cell. 1997 Oct 3;91(1):109-18. doi: 10.1016/s0092-8674(01)80013-1.

Abstract

Three distinct adaptor protein (AP) complexes involved in protein trafficking have been identified. AP-1 and AP-2 mediate protein sorting at the trans-Golgi network and plasma membrane, respectively, whereas the function of AP-3 has not been defined. A screen for factors specifically involved in transport of alkaline phosphatase (ALP) from the Golgi to the vacuole/lysosome has identified Ap16p and Ap15p of the yeast AP-3 complex. Deletion of each of the four AP-3 subunits results in selective mislocalization of ALP and the vacuolar t-SNARE, Vam3p (but not CPS and CPY), while deletion of AP-1 and AP-2 subunits has no effect on vacuolar protein delivery. This study, therefore, provides evidence that the AP-3 complex functions in cargo-selective protein transport from the Golgi to the vacuole/lysosome.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adaptor Protein Complex alpha Subunits
  • Adaptor Proteins, Vesicular Transport
  • Alkaline Phosphatase / genetics
  • Alkaline Phosphatase / metabolism*
  • Biological Transport
  • Carboxypeptidases / metabolism
  • Cathepsin A
  • Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / genetics
  • Dipeptidyl-Peptidases and Tripeptidyl-Peptidases / metabolism
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Fungal Proteins / physiology*
  • Golgi Apparatus / enzymology
  • Lysosomes / enzymology
  • Membrane Proteins / metabolism
  • Membrane Proteins / physiology
  • Monomeric Clathrin Assembly Proteins*
  • Qa-SNARE Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Deletion
  • Vacuoles / enzymology*

Substances

  • Adaptor Protein Complex alpha Subunits
  • Adaptor Proteins, Vesicular Transport
  • Fungal Proteins
  • Membrane Proteins
  • Monomeric Clathrin Assembly Proteins
  • PEP12 protein, S cerevisiae
  • Qa-SNARE Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • VAM3 protein, S cerevisiae
  • clathrin assembly protein AP180
  • Alkaline Phosphatase
  • Carboxypeptidases
  • Dipeptidyl-Peptidases and Tripeptidyl-Peptidases
  • STE13 protein, S cerevisiae
  • Cathepsin A
  • CPS1 protein, S cerevisiae