Cloning of GPR37, a gene located on chromosome 7 encoding a putative G-protein-coupled peptide receptor, from a human frontal brain EST library

Genomics. 1997 Oct 1;45(1):68-77. doi: 10.1006/geno.1997.4900.


A cDNA sequence encoding a putative peptide-specific G-protein-coupled receptor (GPR37) was isolated from a set of human brain frontal lobe expressed sequence tags. The GPR37 cDNA predicts a single open reading frame coding for a 613-amino-acid protein with seven hydrophobic transmembrane domains. The GPR37 genomic sequence was mapped to chromosome 7q31, and it was isolated upon screening of a chromosome 7-specific genomic library. The GPR37 gene spans more than 25 kb and contains two exons and a single intron which interrupts the GPR37 cDNA within the sequence encoding the presumed third transmembrane domain. Northern blot analysis with GPR37 probes revealed a main 3.8-kb mRNA and a less abundant 8-kb mRNA, both expressed in human brain tissues, particularly in corpus callosum, medulla, putamen, and caudate nucleus. The lowest level of expression was detected in cerebellum. The 3.8-kb mRNA is also less abundantly expressed in liver and placenta. Although the ligand for the putative GPR37 receptor has not been identified, its deduced amino acid sequence shows a high degree of homology (approximately 40% in the transmembrane regions) with most mammalian peptide-specific G-protein-coupled receptors and particularly with the human endothelin-B, bombesin-BB1, and bombesin-BB2 receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 7*
  • Cloning, Molecular
  • DNA, Complementary
  • Female
  • Frontal Lobe / metabolism*
  • GTP-Binding Proteins / metabolism
  • Gene Expression
  • Gene Library
  • Humans
  • Membrane Proteins*
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • Receptors, Cell Surface / genetics*
  • Receptors, G-Protein-Coupled*
  • Sequence Homology, Amino Acid


  • DNA, Complementary
  • GPR37 receptor, human
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • GTP-Binding Proteins

Associated data

  • GENBANK/Y12476
  • GENBANK/Y12477