Site-directed mutagenesis: a two-step method using PCR and DpnI

Biotechniques. 1997 Oct;23(4):588-90. doi: 10.2144/97234bm05.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Deoxyribonucleases, Type II Site-Specific / metabolism*
  • Mice
  • Mutagenesis, Site-Directed*
  • Polymerase Chain Reaction / methods*
  • Receptors, Antigen, T-Cell, alpha-beta / genetics

Substances

  • Receptors, Antigen, T-Cell, alpha-beta
  • endodeoxyribonuclease DpnI
  • Deoxyribonucleases, Type II Site-Specific