Dynamic organization of DNA replication in one-cell mouse embryos: relationship to transcriptional activation

Exp Cell Res. 1997 Oct 10;236(1):201-11. doi: 10.1006/excr.1997.3708.

Abstract

We have analyzed the spatial and temporal relationship between transcription and replication sites during the first cell cycle in mouse embryos. Embryos were microinjected with both 5-bromouridine-5'-triphosphate and digoxygenin-11-deoxyuridine-5'-triphosphate to visualize transcription and replication sites respectively. We detected six different phases of replication during S phase and dated the onset of zygotic transcription at the end of the S phase. Using confocal microscopy, we showed that there is essentially no colocalization of replication and transcription sites at this stage of development. Moreover, studies on aphidicolin-treated embryos demonstrated that inhibition of DNA replication does not hinder transcriptional activation at the 1-cell stage.

MeSH terms

  • Animals
  • Aphidicolin / pharmacology
  • Cell Nucleus / physiology
  • DNA / analysis
  • DNA / biosynthesis
  • DNA Replication / physiology*
  • Digoxigenin
  • Enzyme Inhibitors / pharmacology
  • Female
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Microinjections
  • S Phase / physiology
  • Transcription, Genetic / drug effects
  • Transcription, Genetic / physiology*
  • Uridine Triphosphate / analogs & derivatives
  • Uridine Triphosphate / pharmacology
  • Zygote / cytology*
  • Zygote / physiology*

Substances

  • Enzyme Inhibitors
  • 5-bromouridine triphosphate
  • Aphidicolin
  • DNA
  • Digoxigenin
  • Uridine Triphosphate