Molecular cloning and chromosomal localization of PD-Ibeta (PDNP3), a new member of the human phosphodiesterase I genes

Genomics. 1997 Oct 15;45(2):412-5. doi: 10.1006/geno.1997.4949.


Phosphodiesterase I (EC pyrophosphatase (EC enzymes are a family of type II transmembrane proteins that catalyze the cleavage of phosphodiester and phosphosulfate bonds of a variety of molecules, including deoxynucleotides, NAD, and nucleotide sugars. The human genes for two members of this family have been cloned and designated PC-1 (PDNP1) and PD-Ialpha/autotaxin (PDNP2). We have now cloned the third member of this family from a human prostate cDNA library and designated it human phosphodiesterase-Ibeta (PD-Ibeta). The PD-Ibeta cDNA contains a 2625-bp-long open reading frame which encodes an 875-amino-acid protein. COS-7 cells transfected with an expression vector, pBK-CMV, containing PD-Ibeta cDNA had high phosphodiesterase I activity compared to the mock-transfected cells. By using in situ hybridization to human metaphase chromosomes, we have assigned the locus for the PD-Ibeta (PDNP3) gene to the q22 region of human chromosome 6.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 6 / genetics
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Female
  • Gene Expression
  • Humans
  • In Situ Hybridization, Fluorescence
  • Male
  • Molecular Sequence Data
  • Phosphodiesterase I
  • Phosphoric Diester Hydrolases / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Tissue Distribution


  • DNA, Complementary
  • RNA, Messenger
  • Phosphoric Diester Hydrolases
  • Phosphodiesterase I

Associated data

  • GENBANK/AF005632