A high resolution GBG-banded karyotype of the Atlantic bottlenose dolphin, Tursiops truncatus: generation of an ideogram, and NOR localization by fluorescence in situ hybridization

P E Bielec; P K Riggs; J E Womack; D L Busbee

doi:10.1159/000134615

A high resolution GBG-banded karyotype of the Atlantic bottlenose dolphin, Tursiops truncatus: generation of an ideogram, and NOR localization by fluorescence in situ hybridization

Cytogenet Cell Genet. 1997;78(1):6-11. doi: 10.1159/000134615.

Authors

P E Bielec¹, P K Riggs, J E Womack, D L Busbee

Affiliation

¹ Department of Anatomy and Public Health, College of Veterinary Medicine, Texas A&M University, College Station, USA.

PMID: 9345896
DOI: 10.1159/000134615

Abstract

A high resolution karyotype was prepared using GBG-banded chromosomes from kidney epithelial cell lines of the Atlantic bottlenose dolphin, Tursiops truncatus. The karyotype was used to generate a banded ideogram of T. truncatus that will facilitate cytogenetic analyses essential for comparison of dolphin chromosomes with those of potentially related terrestrial vertebrates. Fluorescence in situ hybridization analysis of the karytype using a rodent (Geomys bursarius) 28S ribosomal DNA (rDNA) probe allowed localization of four nucleolar organizer (NOR) regions to the short arms of two chromosome pairs. FISH mapping, using DNA probes, is dependent on a pre-existing banded ideogram, and provides the means to initiate physical mapping of the dolphin genome.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Cell Line
Chromosome Banding
Chromosome Mapping*
Dolphins / genetics*
Female
In Situ Hybridization, Fluorescence
Karyotyping
Kidney / cytology
Metaphase
Nucleolus Organizer Region / genetics*