The homeobox gene GBX2, a target of the myb oncogene, mediates autocrine growth and monocyte differentiation

Cell. 1997 Oct 17;91(2):185-95. doi: 10.1016/s0092-8674(00)80401-8.


The homeobox gene GBX2 was identified as a target gene of the v-Myb oncoprotein encoded by the avian myeloblastosis virus (AMV). GBX2 activation by c-Myb requires signal transduction emanating from the cell surface while the leukemogenic AMV v-Myb constitutively induces the GBX2 gene. Mutations in the DNA binding domain of AMV-Myb render it independent of signaling events and concomitantly abrogate the collaboration between Myb and CCAAT Enhancer Binding Proteins (C/EBP), which are involved in granulocyte differentiation. Ectopic expression of GBX2 in growth factor-dependent myeloblasts induces monocytic features and independence from exogenous cytokines, reflecting distinct features of AMV-transformed cells. Our results suggest that Myb or factors it interacts with contribute to hematopoietic lineage choice and differentiation in a signal transduction-dependent fashion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autocrine Communication / physiology*
  • Avian Proteins*
  • Bone Marrow Cells / chemistry
  • Bone Marrow Cells / physiology
  • Cell Differentiation / genetics
  • Chickens
  • Cytokines
  • DNA-Binding Proteins / physiology
  • Gene Expression Regulation, Developmental / physiology
  • Growth Substances / genetics
  • Growth Substances / metabolism
  • Hematopoiesis / genetics
  • Hematopoietic Stem Cells / chemistry
  • Hematopoietic Stem Cells / physiology
  • Homeodomain Proteins / genetics*
  • Homeodomain Proteins / metabolism*
  • Intercellular Signaling Peptides and Proteins*
  • Molecular Sequence Data
  • Monocytes / cytology*
  • Oncogenes / physiology*
  • Phenotype
  • Promoter Regions, Genetic / physiology
  • Signal Transduction / genetics
  • Transformation, Genetic


  • Avian Proteins
  • Cytokines
  • DNA-Binding Proteins
  • Growth Substances
  • Homeodomain Proteins
  • Intercellular Signaling Peptides and Proteins
  • MGF protein, chicken

Associated data

  • GENBANK/AF022151