Mouse germinal center B cells with the xid mutation retain responsiveness to antimouse CD40 antibodies but diminish IL-5 responsiveness

Int Immunol. 1997 Oct;9(10):1463-73. doi: 10.1093/intimm/9.10.1463.


The germinal center (GC) develops in secondary lymphoid tissues in response to thymus-dependent (TD) antigens. To investigate the molecular mechanism of B cell differentiation in GC, we enriched GC B cells from spleen of TD antigen-immunized wild-type and X-linked immunodeficient (XID) mice, and examined the differentiation of GC B cells into antigen-specific IgG1 antibody-forming cells (AFC) in response to anti-CD40 mAb and cytokines. A significant proportion of freshly purified GC B cells expressed receptors for IL-4 and IL-5. Anti-CD40 mAb sustained the viability of GC B cells and IL-4 co-operated with anti-CD40 mAb for further enhancement of the cell viability. Anti-CD40 mAb and IL-4 were essential for inducing differentiation of GC B cells into antigen-specific IgG1-AFC and IL-5 efficiently enhanced their differentiation. GC B cells with the xid mutation responded for proliferation to CD40 ligation to a lesser extent and for the IgG1-AFC response to anti-CD40 mAb together with IL-4, but they showed impaired responsiveness to IL-5, regardless of enhanced expression of IL-5R in response to anti-CD40 mAb and IL-4. These results suggest that anti-CD40 mAb, IL-4 and IL-5 play a critical role in the differentiation of mouse GC B cells. The GC B cells from XID mice show a functional defect with respect to IL-5-mediated differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / pathology
  • CD40 Antigens / immunology
  • Cell Differentiation
  • Cell Survival
  • Female
  • Germinal Center / immunology*
  • Germinal Center / pathology
  • Immunoglobulin G / biosynthesis
  • Immunologic Deficiency Syndromes / genetics*
  • Immunologic Deficiency Syndromes / immunology*
  • Immunologic Deficiency Syndromes / pathology
  • Interleukin-5 / immunology
  • Mice
  • Mice, Inbred BALB C
  • Mice, Mutant Strains
  • Mutation
  • Phenotype
  • Receptors, Interleukin / metabolism
  • Receptors, Interleukin-5
  • X Chromosome


  • Antibodies, Monoclonal
  • CD40 Antigens
  • Immunoglobulin G
  • Interleukin-5
  • Receptors, Interleukin
  • Receptors, Interleukin-5