Gene expression systems in the development of high-throughput screens

Curr Opin Biotechnol. 1997 Oct;8(5):629-34. doi: 10.1016/s0958-1669(97)80040-7.

Abstract

Recent advances in the development of combinatorial automated chemical synthesis, robotic sample handling, and data collection and analysis have significantly increased the number of compounds available for screening against potential therapeutic targets. The implementation of highly sensitive in vitro biochemical and cell-based high-throughput screening assays is essential to facilitate the rapid identification of selective and potent lead molecules from compound libraries. The ability to easily produce functional proteins in sufficient quantities for in vitro biochemical assays and to devise useful cell-based systems is dependent on the successful application of a variety of gene expression systems.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Culture Techniques / methods
  • Cell Line
  • Cloning, Molecular / methods
  • Cytochrome P-450 Enzyme System / biosynthesis
  • Gene Expression*
  • Ligands
  • Mammals
  • Melanophores / cytology
  • Receptors, Cell Surface / biosynthesis
  • Receptors, Cell Surface / physiology
  • Recombinant Proteins / biosynthesis*
  • Saccharomyces cerevisiae
  • Transfection / methods
  • Xenobiotics / metabolism
  • Xenopus laevis

Substances

  • Ligands
  • Receptors, Cell Surface
  • Recombinant Proteins
  • Xenobiotics
  • Cytochrome P-450 Enzyme System