Identification and cloning of differentially expressed genes

Curr Opin Biotechnol. 1997 Oct;8(5):542-6. doi: 10.1016/s0958-1669(97)80026-2.

Abstract

Only a few of the methods currently used for identification of differentially expressed genes take advantage of the fact that (near) complete sets of cDNA clones and sequences representing all human and mouse genes will be available for high throughput survey of gene expression. Accordingly, strategies based on hybridization of complex (cDNA or RNA) probes to cDNA microarrays, either on glass slides or on chips, are likely to become increasingly more advantageous. Recognizing, however, that the power of these methods depends upon the availability of such resources, strategies are being pursued to facilitate completion of the ongoing efforts to identify all human and mouse genes.

Publication types

  • Review

MeSH terms

  • Animals
  • Cloning, Organism / methods*
  • DNA, Complementary
  • Gene Expression*
  • Gene Library
  • Humans
  • Mice
  • Polymerase Chain Reaction / methods
  • RNA Probes

Substances

  • DNA, Complementary
  • RNA Probes