Specification of mouse telencephalic and mid-hindbrain progenitors following heterotopic ultrasound-guided embryonic transplantation

Neuron. 1997 Oct;19(4):761-72. doi: 10.1016/s0896-6273(00)80959-9.


We have demonstrated the utility of ultrasound backscatter microscopy for targeted intraparenchymal injections into embryonic day (E) 13.5 mouse embryos. This system has been used to test the degree of commitment present in neural progenitors from the embryonic ventral telencephalon and mid-hindbrain region. Many E13.5 ventral telencephalic progenitors were observed to integrate and adopt local phenotypes following heterotopic transplantation into telencephalic or mid-hindbrain targets, whereas mid-hindbrain cells of the same stage were unable to integrate and change fate in the telencephalon. In contrast, many mid-hindbrain cells from an earlier developmental stage (E10.5) were capable of integrating and adopting a forebrain phenotype after grafting into the telencephalon, suggesting that mouse mid-hindbrain progenitors become restricted in their developmental potential between E10.5 and E13.5.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Tissue Transplantation / methods
  • Brain Tissue Transplantation / physiology*
  • Cell Differentiation
  • Fetal Tissue Transplantation / methods
  • Fetal Tissue Transplantation / physiology*
  • Mice
  • Mice, Inbred Strains
  • Mice, Transgenic
  • Neurons / cytology
  • Neurons / physiology
  • Neurons / transplantation
  • Prosencephalon / diagnostic imaging
  • Prosencephalon / physiology*
  • Rhombencephalon / cytology
  • Rhombencephalon / physiology*
  • Rhombencephalon / transplantation
  • Stem Cells / cytology
  • Stem Cells / physiology*
  • Telencephalon / cytology
  • Telencephalon / physiology*
  • Telencephalon / transplantation
  • Transplantation, Heterotopic / methods
  • Transplantation, Heterotopic / physiology
  • Ultrasonography / methods
  • beta-Galactosidase / biosynthesis


  • beta-Galactosidase