Alterations of the phenotype of colonic macrophages in inflammatory bowel disease

Eur J Gastroenterol Hepatol. 1997 Sep;9(9):893-9. doi: 10.1097/00042737-199709000-00013.

Abstract

Background: Intestinal macrophages play an important role in mucosal inflammation. In normal colonic mucosa we recently demonstrated a unique macrophage phenotype with attenuated immune functions. Here we present an analysis of the alterations of the phenotype of colonic macrophages in inflammatory bowel disease (IBD).

Methods: Intestinal macrophages were isolated from biopsies of patients with IBD (n =20). Flow cytometric triple fluorescence analysis was applied to study CD14, CD16, CD33, HLA-DR, CD44, CD11b, CD11c and CD3/CD19 expression.

Results: In IBD there was an increase in expression not only of CD14 compared to control mucosa (36.0% +/- 13.2% vs. 10.5% +/- 3.8%, P< 0.0001) but also of CD16 (28.6% +/- 10.3% vs. 10.1% +/- 3.9%, P< 0.0001), HLA-DR (53.1% +/- 15.9% vs. 27.3% +/- 9.2%, P< 0.0005), CD11b (42.8% +/- 14.2% vs. 17.4% +/- 6.8%, P< 0.0001) and CD11c (35.1% +/- 15.9% vs. 17.8% +/- 10.4%, P< 0.005.). Furthermore, a hitherto undescribed new population of macrophages could be detected by flow cytometry only in patients with ulcerative colitis (CD16++, CD11b++, CD14(low), CD33(low), CD11c-) accounting for 5.8% of all cells isolated.

Conclusion: In contrast to colonic macrophages from normal mucosa, there is a significantly higher expression of CD14, CD16, HLA-DR, CD11b and CD11c in IBD, indicating additional macrophage populations in the inflamed mucosa. This may reflect either a recruitment of new cells from the circulation or a change in phenotype of resident cells.

MeSH terms

  • Antigens, CD / analysis*
  • Antigens, Differentiation, Myelomonocytic / analysis
  • Biopsy
  • Cell Adhesion Molecules / analysis
  • Colitis, Ulcerative / immunology*
  • Colon / chemistry
  • Colon / immunology*
  • Colon / pathology*
  • Crohn Disease / immunology*
  • Flow Cytometry / methods
  • HLA-DR Antigens / analysis
  • Humans
  • Hyaluronan Receptors / analysis
  • Integrin alphaXbeta2 / analysis
  • Intestinal Mucosa / chemistry
  • Intestinal Mucosa / immunology*
  • Lipopolysaccharide Receptors / analysis
  • Macrophage-1 Antigen / analysis
  • Macrophages / chemistry
  • Macrophages / pathology*
  • Receptors, IgG / analysis
  • Sialic Acid Binding Ig-like Lectin 3

Substances

  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD33 protein, human
  • Cell Adhesion Molecules
  • HLA-DR Antigens
  • Hyaluronan Receptors
  • Integrin alphaXbeta2
  • Lipopolysaccharide Receptors
  • Macrophage-1 Antigen
  • Receptors, IgG
  • Sialic Acid Binding Ig-like Lectin 3