Identification of three allatostatins and their cDNA from the mosquito Aedes aegypti

Peptides. 1997;18(7):937-42. doi: 10.1016/s0196-9781(97)00032-6.

Abstract

Three allatostatins have been isolated from the mosquito Aedes aegypti. These peptides have the following structures: Ser-Pro-Lys-Tyr-Asn-Phc-Gly-Leu-amide, Leu-Pro-His-Tyr-Asn-Phe-Gly-Leu-amide, and Arg-Val-Tyr-Asp-Phe-Gly-Leu-amide. A cDNA encoding these peptides was isolated from an abdominal ganglia cDNA library and sequenced. It was found to encode two additional allatostatins: Ala-Ser-Ala-Tyr-Arg-Tyr-His-Phe-Gly-Leu-amide and Leu-Pro-Asn-Arg-Tyr-Asn-Phe-Gly-Leu-amide. Northern analysis of whole mosquito mRNA revealed a single prepro-allatostatin message of around 3,000 bases. Identification of a partial prepro-allatostatin cDNA from a midgut cDNA library shows that the same gene is also expressed in the mosquito midgut.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aedes / genetics*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Complementary / genetics*
  • Molecular Sequence Data
  • Neuropeptides / genetics*
  • RNA, Messenger / genetics
  • Restriction Mapping

Substances

  • DNA Primers
  • DNA, Complementary
  • Neuropeptides
  • RNA, Messenger
  • allatostatin