Direct cloning of the Brassica S locus by using a P1-derived artificial chromosome (PAC) vector

Gene. 1997 Oct 15;199(1-2):133-7. doi: 10.1016/s0378-1119(97)00358-2.


Self-incompatibility of Brassica is regulated by the S locus, which contains several genes including SLG and SRK. We found that both SLG and SRK genes were located at an approx. 80-kb MluI fragment in an S9 haplotype of B. campestris. Therefore, we cloned this MluI fragment into a BssHII site of the P1-derived artificial chromosome (PAC) vector. The utility of the direct cloning method is discussed in this study.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / genetics
  • Bacterial Proteins*
  • Brassica / genetics*
  • Cloning, Molecular / methods*
  • Deoxyribonucleases, Type II Site-Specific
  • Genes, Plant / genetics*
  • Genetic Vectors / genetics*
  • Glycoproteins / genetics*
  • Plant Proteins / genetics*
  • Plasmids / genetics
  • Protein Kinases / genetics


  • Bacterial Proteins
  • Glycoproteins
  • Plant Proteins
  • self-incompatibility locus specific glycoprotein, Brassica
  • Protein Kinases
  • S-receptor kinase
  • endodeoxyribonuclease MluI
  • Deoxyribonucleases, Type II Site-Specific