Abstract
We report the molecular cloning, chromosome mapping and developmental transcription pattern of a putative serine/threonine protein phosphatase 2C (PP2C), DPP2C1, from Drosophila melanogaster. The 6-kb transcript of this first Drosophila PP2C gene encodes a 1428-aa deduced protein. The DPP2C1 protein contains a approximately 330-aa PP2C-like catalytic domain flanked by extensive N- and C-terminal sequences showing no similarities to other PP2Cs. The dpp2c1 gene maps to 4E1-2 on the X chromosome, 1.5 kb upstream of the ddlc1 gene. Northern blot analyses showed that dpp2c1 transcription is developmentally regulated, accumulating maximally during early (0-6 h) and late (12-24 h) embryogensis. The presented molecular characterisation provides the basis for a genetic dissection of DPP2C1 function.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Binding Sites
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Chromosome Mapping
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Cloning, Molecular
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DNA, Complementary / genetics
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Drosophila melanogaster / embryology
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Drosophila melanogaster / genetics*
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Gene Expression Regulation, Developmental / physiology*
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Genes, Insect / genetics
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Metals / metabolism
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Molecular Sequence Data
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Phosphoprotein Phosphatases / genetics*
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Protein Phosphatase 2
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Protein Phosphatase 2C
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RNA, Messenger / analysis
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Restriction Mapping
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Saccharomyces cerevisiae Proteins*
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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X Chromosome / genetics
Substances
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DNA, Complementary
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Metals
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RNA, Messenger
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Saccharomyces cerevisiae Proteins
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PTC1 protein, S cerevisiae
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Phosphoprotein Phosphatases
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Protein Phosphatase 2
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Protein Phosphatase 2C