Drosophila DPP2C1, a Novel Member of the Protein Phosphatase 2C (PP2C) Family

Gene. 1997 Oct 15;199(1-2):139-43. doi: 10.1016/s0378-1119(97)00359-4.


We report the molecular cloning, chromosome mapping and developmental transcription pattern of a putative serine/threonine protein phosphatase 2C (PP2C), DPP2C1, from Drosophila melanogaster. The 6-kb transcript of this first Drosophila PP2C gene encodes a 1428-aa deduced protein. The DPP2C1 protein contains a approximately 330-aa PP2C-like catalytic domain flanked by extensive N- and C-terminal sequences showing no similarities to other PP2Cs. The dpp2c1 gene maps to 4E1-2 on the X chromosome, 1.5 kb upstream of the ddlc1 gene. Northern blot analyses showed that dpp2c1 transcription is developmentally regulated, accumulating maximally during early (0-6 h) and late (12-24 h) embryogensis. The presented molecular characterisation provides the basis for a genetic dissection of DPP2C1 function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Gene Expression Regulation, Developmental / physiology*
  • Genes, Insect / genetics
  • Metals / metabolism
  • Molecular Sequence Data
  • Phosphoprotein Phosphatases / genetics*
  • Protein Phosphatase 2
  • Protein Phosphatase 2C
  • RNA, Messenger / analysis
  • Restriction Mapping
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • X Chromosome / genetics


  • DNA, Complementary
  • Metals
  • RNA, Messenger
  • Saccharomyces cerevisiae Proteins
  • PTC1 protein, S cerevisiae
  • Phosphoprotein Phosphatases
  • Protein Phosphatase 2
  • Protein Phosphatase 2C

Associated data

  • GENBANK/U96697