Abstract
The isolation and structure elucidation of five novel natural Diels--Alder-type adducts, named palodesangrens A-E (1-5), from the Peruvian folk medicine known as "palo de sangre" (Brosimum rubescens) is described. The structures of the Diels--Alder adducts, consisting of chalcone derivatives and a prenylcoumarin, were elucidated by analysis of spectroscopic data including 2D NMR. Some of these compounds showed potent inhibitory activity towards 5 alpha-dihydrotestosterone (DHT) binding with an androgen receptor to form a DHT-receptor complex that causes androgen-dependent diseases.
MeSH terms
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5-alpha Reductase Inhibitors
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Androgen Antagonists / chemistry
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Androgen Antagonists / isolation & purification*
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Androgen Antagonists / pharmacology
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Androgen Receptor Antagonists
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Animals
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Chalcone / analogs & derivatives
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Chalcone / chemistry
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Chalcone / isolation & purification*
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Chalcone / pharmacology
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Chromatography, High Pressure Liquid
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Coumarins / chemistry
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Coumarins / isolation & purification*
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Coumarins / pharmacology
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In Vitro Techniques
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Magnetic Resonance Spectroscopy
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Male
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Peru
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Plant Epidermis / chemistry
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Plants, Medicinal / chemistry*
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Rats
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Rats, Wistar
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Spectrometry, Mass, Fast Atom Bombardment
Substances
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5-alpha Reductase Inhibitors
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Androgen Antagonists
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Androgen Receptor Antagonists
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Coumarins
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Chalcone