The DNA damage-recognition problem in human and other eukaryotic cells: the XPA damage binding protein

Biochem J. 1997 Nov 15;328 ( Pt 1)(Pt 1):1-12. doi: 10.1042/bj3280001.

Abstract

The capacity of human and other eukaryotic cells to recognize a disparate variety of damaged sites in DNA, and selectively excise and repair them, resides in a deceptively small simple protein, a 38-42 kDa zinc-finger binding protein, XPA (xeroderma pigmentosum group A), that has no inherent catalytic properties. One key to its damage-recognition ability resides in a DNA-binding domain which combines a zinc finger and a single-strand binding region which may infiltrate small single-stranded regions caused by helix-destabilizing lesions. Another is the augmentation of its binding capacity by interactions with other single-stranded binding proteins and helicases which co-operate in the binding and are unloaded at the binding site to facilitate further unwinding of the DNA and subsequent catalysis. The properties of these reactions suggest there must be considerable conformational changes in XPA and associated proteins to provide a flexible fit to a wide variety of damaged structures in the DNA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • DNA Damage*
  • DNA Repair*
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • Eukaryotic Cells / metabolism*
  • Gene Expression Regulation
  • Humans
  • Models, Genetic
  • Mutation
  • Substrate Specificity / genetics
  • Xeroderma Pigmentosum / genetics*
  • Xeroderma Pigmentosum Group A Protein

Substances

  • DNA-Binding Proteins
  • XPA protein, human
  • Xeroderma Pigmentosum Group A Protein