CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment

Cell. 1997 Oct 31;91(3):357-66. doi: 10.1016/s0092-8674(00)80419-5.


Mitosis requires dynamic attachment of chromosomes to spindle microtubules. This interaction is mediated largely by kinetochores. During prometaphase, forces exerted at kinetochores, in combination with polar ejection forces, drive congression of chromosomes to the metaphase plate. A major question has been whether kinetochore-associated microtubule motors play an important role in congression. Using immunodepletion from and antibody addition to Xenopus egg extracts, we show that the kinetochore-associated kinesin-like motor protein CENP-E is essential for positioning chromosomes at the metaphase plate. We further demonstrate that CENP-E powers movement toward microtubule plus ends in vitro. These findings support a model in which CENP-E functions in congression to tether kinetochores to dynamic microtubule plus ends.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies / immunology
  • Base Sequence
  • Centromere / physiology
  • Chromosomal Proteins, Non-Histone / immunology
  • Chromosomal Proteins, Non-Histone / physiology*
  • Chromosomes / physiology
  • Humans
  • Kinetochores / physiology*
  • Metaphase*
  • Molecular Sequence Data
  • Xenopus


  • Antibodies
  • Chromosomal Proteins, Non-Histone
  • centromere protein E

Associated data

  • GENBANK/AF027728