Transcytosis and surface presentation of IL-8 by venular endothelial cells

Cell. 1997 Oct 31;91(3):385-95. doi: 10.1016/s0092-8674(00)80422-5.


Chemokines have been convincingly implicated in actuating inflammatory leukocyte emigration. To affect the circulating leukocytes, tissue-derived chemokines have to traverse the endothelial cells (ECs). This was thought to be accomplished by chemokine diffusion through the intercellular gaps. On the contrary, we show by electron microscopy that the prototype chemokine IL-8 is internalized by venular ECs abluminally and transcytosed to the luminal surface. Here, it is presented to the adherent leukocytes on the EC membrane, predominantly in association with the EC projections. The intact C terminus of IL-8, the molecule's "immobilization" domain, is required for the EC binding, transcytosis, and consequently, the in vivo proemigratory activity of IL-8, indicating that the described subcellular interactions of IL-8 with the ECs are functionally relevant.

MeSH terms

  • Animals
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / metabolism*
  • Humans
  • Interleukin-8 / metabolism*
  • Interleukin-8 / pharmacology
  • Macaca mulatta
  • Mice
  • Mice, Inbred BALB C
  • Neutrophils / cytology
  • Neutrophils / metabolism
  • Rabbits
  • Recombinant Proteins / metabolism
  • Recombinant Proteins / pharmacology
  • Venules / cytology
  • Venules / metabolism


  • Interleukin-8
  • Recombinant Proteins