Cloning, expression, and chromosomal mapping of a novel human CC-chemokine receptor (CCR10) that displays high-affinity binding for MCP-1 and MCP-3

DNA Cell Biol. 1997 Oct;16(10):1249-56. doi: 10.1089/dna.1997.16.1249.


Chemokines mediate their chemotactic, proinflammatory effects by binding to and activating a variety of specific receptors belonging to the G protein-coupled superfamily of seven-transmembrane serpentine receptors. We report the cloning, chromosomal localization, expression, and ligand binding of a novel CC chemokine receptor, CCR10. CCR10 is expressed primarily in placenta and fetal liver, and binds two of the CC chemokines, monocyte chemoattractant protein (MCP)-1 and MCP-3, with highest affinity. The KD for MCP-3 binding was 1 nM, and MCP-1 competed for MCP-3 binding with an IC50 of 1.2 nM. The CC chemokines MCP-4 and RANTES competed for MCP-3 binding with IC50 values of 7.5 and 5.4 nM, respectively. The chromosomal location of CCR10 was determined to coincide with the CC chemokine receptor cluster on chromosome 3 (3p21.31-3p21.32). These results indicate that CCR10 is a novel CC chemokine receptor with a unique expression pattern that would be consistent with a role in placental immunity or hematopoiesis.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding, Competitive
  • COS Cells
  • Chemokine CCL2 / metabolism*
  • Chemokine CCL5 / metabolism
  • Chemokine CCL7
  • Chromosome Mapping
  • Chromosomes, Human, Pair 3 / genetics
  • Cloning, Molecular
  • Cytokines*
  • Female
  • Gene Expression Regulation, Developmental / physiology*
  • Humans
  • Kinetics
  • Male
  • Molecular Sequence Data
  • Monocyte Chemoattractant Proteins / metabolism*
  • Organ Specificity
  • RNA, Messenger / analysis
  • Receptors, CCR10
  • Receptors, Chemokine / genetics*
  • Receptors, Chemokine / metabolism*
  • Sequence Homology, Amino Acid


  • CCL7 protein, human
  • CCR10 protein, human
  • Chemokine CCL2
  • Chemokine CCL5
  • Chemokine CCL7
  • Cytokines
  • Monocyte Chemoattractant Proteins
  • RNA, Messenger
  • Receptors, CCR10
  • Receptors, Chemokine

Associated data

  • GENBANK/U94888