Double staining technique for rat foetus skeletons in teratological studies

Lab Anim. 1979 Oct;13(4):309-10. doi: 10.1258/002367779780943233.

Abstract

Cartilage and bone were differentiated using alcian blue and alizarin red S respectively. Anomalies of both cartilaginous and bony parts of the skeleton could be examined.

MeSH terms

  • Alcian Blue
  • Animals
  • Anthraquinones
  • Arylsulfonates
  • Bone and Bones / abnormalities*
  • Cartilage / abnormalities*
  • Coloring Agents
  • Female
  • Fetus
  • Pregnancy
  • Rats / embryology*
  • Rodent Diseases / congenital
  • Rodent Diseases / embryology*
  • Staining and Labeling*

Substances

  • Anthraquinones
  • Arylsulfonates
  • Coloring Agents
  • Alizarin Red S
  • Alcian Blue