Evidence for oxidative stress in the hepatic mitochondria of bile duct ligated rats

Pharmacol Res. 1997 Sep;36(3):243-7. doi: 10.1006/phrs.1997.0225.


Lipid peroxidation increased both in the liver homogenates and in the hepatic mitochondrial fraction of bile duct ligated (BDL)-rats. Although mitochondrial superoxide dismutase (SOD) activity did not change in the liver, glutathione (GSH) levels and glutathione peroxidase (GSH-Px) activity decreased in hepatic mitochondrial fraction of BDL-rats as compared to sham-operated rats. In addition, GSH-Px and glutathione transferase (GST) activities decreased but SOD activity remained unchanged in the post-mitochondrial fraction of the liver from BDL-rats. However, erythrocyte lipid peroxide and GSH levels did not change in BDL-rats. In conclusion, our results show that the disturbance of oxidant-antioxidant balance especially in mitochondria may be responsible for cholestatic liver injury in BDL-rats.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cholestasis / metabolism*
  • Erythrocytes / metabolism
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • Lipid Peroxidation / physiology
  • Liver / metabolism*
  • Male
  • Mitochondria, Liver / metabolism*
  • Oxidative Stress / physiology*
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / metabolism


  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione