Suppression of cytochrome P450 Cyp2f2 mRNA levels in mice by the peroxisome proliferator diethylhexylphthalate

Biochem Biophys Res Commun. 1997 Oct 29;239(3):660-5. doi: 10.1006/bbrc.1997.7529.


Exposure to peroxisome proliferators, which are extensively used, causes a number of pleiotrophic effects. Prolonged exposure to the peroxisome proliferator, DEHP, causes hepatic hyperplasia and liver tumors in rats and mice. This exposure can also induce a number of enzymes. To identify additional genes that are regulated by DEHP, mRNA differential display was used. One of the genes affected is cytochrome 450 Cyp2f2, a naphthalene hydroxylase. Using northern analysis, RNase protection assay, and RT-PCR, we show that the Cyp2f2 mRNA levels are decreased in mouse liver following DEHP treatment. A smaller Cyp2f2 mRNA transcript was also detected in kidney and these transcript levels were also suppressed but to a lesser extent than that in the liver. The response to DEHP in mouse liver is both dose and time dependent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cytochrome P-450 Enzyme System / biosynthesis*
  • Cytochrome P-450 Enzyme System / drug effects
  • Cytochrome P-450 Enzyme System / genetics*
  • Diethylhexyl Phthalate / pharmacology*
  • Dose-Response Relationship, Drug
  • Enzyme Repression / drug effects
  • Enzyme Repression / genetics
  • Gene Expression Regulation, Enzymologic / drug effects
  • Kidney / enzymology
  • Liver / enzymology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Microbodies / drug effects
  • Microbodies / enzymology*
  • Molecular Sequence Data
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / drug effects*


  • RNA, Messenger
  • Cytochrome P-450 Enzyme System
  • Diethylhexyl Phthalate