The generation of 1H-NMR-detectable mobile lipid in stimulated lymphocytes: relationship to cellular activation, the cell cycle, and phosphatidylcholine-specific phospholipase C

Biochem Biophys Res Commun. 1997 Oct 29;239(3):868-74. doi: 10.1006/bbrc.1997.7566.

Abstract

Mobile lipids detected using 1H-NMR in stimulated lymphocytes were correlated with cell cycle phase, expression of the interleukin-2 receptor alpha and proliferation to assess the activation status of the lymphocytes. Mobile lipid levels, IL-2R alpha expression and proliferation increased after treatment with PMA and ionomycin. PMA or ionomycin stimulation alone induced increased IL-2R alpha expression but not proliferation. PMA- but not ionomycin-stimulation generated mobile lipid. Treatment with anti-CD3 antibody did not increase IL-2R alpha expression or proliferation but did generate increased amounts of mobile lipid. The cell cycle status of thymocytes treated with anti-CD3, PMA or ionomycin alone indicated an accumulation of the cells in the G1 phase of the cell cycle. The generation of mobile lipid was abrogated in anti-CD3 antibody-stimulated thymic lymphocytes but not in splenic lymphocytes, using a phosphatidylcholine-specific phospholipase C (PC-PLC) inhibitor which blocked cells in the G1/S phase of the cell cycle. This suggests that the 1H-NMR-detectable mobile lipid may be generated in anti-CD3 antibody-stimulated thymic lymphocytes by the action of PC-PLC activity via the catabolism of PC, in the absence of classical signs of activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Bridged-Ring Compounds / pharmacology
  • CD3 Complex / immunology
  • Cell Cycle / immunology*
  • Cell Division / drug effects
  • Cell Division / immunology
  • Cell Survival / drug effects
  • Cell Survival / immunology
  • Drug Combinations
  • Ionomycin / pharmacology
  • Lipid Metabolism
  • Lipids / biosynthesis*
  • Lymphocyte Activation*
  • Lymphocytes / immunology
  • Lymphocytes / metabolism*
  • Magnetic Resonance Spectroscopy
  • Mice
  • Mice, Inbred C57BL
  • Phosphatidylcholines / metabolism*
  • Receptors, Interleukin-2 / biosynthesis
  • Receptors, Interleukin-2 / immunology
  • Spleen / cytology
  • Spleen / enzymology
  • Spleen / immunology
  • T-Lymphocytes / enzymology
  • T-Lymphocytes / immunology
  • Tetradecanoylphorbol Acetate / pharmacology
  • Thiones / pharmacology
  • Thymus Gland / cytology
  • Thymus Gland / enzymology
  • Thymus Gland / immunology
  • Type C Phospholipases / antagonists & inhibitors
  • Type C Phospholipases / metabolism*
  • Up-Regulation / drug effects
  • Up-Regulation / immunology

Substances

  • Antibodies, Monoclonal
  • Bridged-Ring Compounds
  • CD3 Complex
  • Drug Combinations
  • Lipids
  • Phosphatidylcholines
  • Receptors, Interleukin-2
  • Thiones
  • Ionomycin
  • tricyclodecane-9-yl-xanthogenate
  • Type C Phospholipases
  • phosphatidylcholine-specific phospholipase C
  • Tetradecanoylphorbol Acetate