Temporal and spatial specificity of PDGF alpha receptor promoter in transgenic mice

Gene Expr. 1997;6(5):301-14.


Aberrant expression of the platelet-derived growth factor alpha receptor (PDGF alpha R) has been linked to developmental abnormalities in vertebrate models, and has been implicated in multiple disease states in humans. To identify cis-acting regulatory elements that dictate expression of this receptor, we generated transgenic mice bearing the reporter gene beta-galactosidase (lacZ) under the control of a 6-kb promoter sequence. Expression of lacZ was monitored throughout embryonic development, with special focus on nervous tissue, skeleton, and several organ systems wherein PDGF alpha R expression is thought to play a pivotal role. In several independent transgenic mouse strains, lacZ expression recapitulated predominant features of PDGF alpha R gene expression during mouse development. These results demonstrate that critical tissue-specific regulatory elements for PDGF alpha R expression are located within a 6-kb upstream region of the PDGF alpha R gene.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bone and Bones / embryology
  • Bone and Bones / metabolism
  • Embryonic and Fetal Development
  • Gastrula
  • Gene Expression
  • Gene Expression Regulation, Developmental
  • Lac Operon
  • Mice
  • Mice, Transgenic
  • Nervous System / embryology
  • Nervous System / metabolism
  • Promoter Regions, Genetic*
  • Receptors, Platelet-Derived Growth Factor / biosynthesis
  • Receptors, Platelet-Derived Growth Factor / genetics*
  • Receptors, Platelet-Derived Growth Factor / metabolism
  • Recombinant Fusion Proteins


  • Recombinant Fusion Proteins
  • Receptors, Platelet-Derived Growth Factor