Identification and analysis of a novel member of the ubiquitin family expressed in dendritic cells and mature B cells

Eur J Immunol. 1997 Oct;27(10):2471-7. doi: 10.1002/eji.1830271002.


Using a cDNA subtraction technique, a novel member of the ubiquitin family was isolated from human dendritic cells. This gene encodes a diubiquitin protein containing tandem head to tail ubiquitin-like domains, with the conservation of key functional residues. Expression of this 777-bp mRNA was restricted to dendritic cells and B cells, with strong expression in mature B cells. Southern blot analysis indicated that a single copy of this gene is present. In situ hybridization on tonsillar tissue showed expression in epithelial cells and isolated cells within the germinal center. With respect to an expressed-sequence tag (EST) this cDNA could be localized to the major histocompatibility complex class I region of chromosome 6. Comparative analysis and the expression pattern of this gene suggests a function in antigen processing and presentation.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • B-Lymphocytes / metabolism*
  • Base Sequence
  • Cell Differentiation
  • Consensus Sequence
  • DNA, Complementary / genetics
  • Dendritic Cells / metabolism*
  • Fetal Blood / cytology
  • Gene Expression
  • Genes
  • Humans
  • Molecular Sequence Data
  • Palatine Tonsil / cytology
  • Polymerase Chain Reaction
  • RNA, Messenger / biosynthesis
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Subtraction Technique
  • Ubiquitins / biosynthesis
  • Ubiquitins / genetics
  • Ubiquitins / isolation & purification*


  • DNA, Complementary
  • RNA, Messenger
  • UBD protein, human
  • Ubiquitins