Human recombinant interferon-beta influences T helper subset differentiation by regulating cytokine secretion pattern and expression of homing receptors

Eur J Immunol. 1997 Oct;27(10):2650-6. doi: 10.1002/eji.1830271026.

Abstract

Type I interferons (IFN) are important regulators of both innate and acquired immunity. We have used an in vitro system of human CD4+ T cell differentiation to determine how IFN-beta influences development of T helper (Th) subsets and homing receptor expression. IFN-beta promoted differentiation of CD4+ T cells that produce low levels of both IFN-gamma and lymphotoxin compared to interleukin (IL)-12-derived Th1 CD4+ T cells. IFN-beta inhibited production of Th2 cytokines (IL-5 and IL-13) and augmented IL-12-mediated IL-10 secretion. In addition, IFN-beta significantly enhanced L-selection expression on CD4+ T cells and synergized with IL-12 to induce expression of cutaneous lymphocyte-associated antigen (CLA). This Th1 L-selectin+, CLA+ phenotype is characteristic of T cells found in normal human skin and suggests a role for type I IFN in the regulation of Th subset differentiation and tissue-specific homing receptors.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Neoplasm
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Gene Expression Regulation / drug effects*
  • Humans
  • Interferon-beta / pharmacology*
  • Interleukin-10 / biosynthesis
  • Interleukin-10 / genetics
  • Interleukin-10 / metabolism
  • Interleukin-12 / pharmacology
  • Interleukin-13 / biosynthesis
  • Interleukin-13 / genetics
  • Interleukin-13 / metabolism
  • Interleukin-5 / biosynthesis
  • Interleukin-5 / genetics
  • Interleukin-5 / metabolism
  • L-Selectin / biosynthesis
  • L-Selectin / genetics
  • Lymphokines / biosynthesis*
  • Lymphokines / genetics
  • Lymphokines / metabolism
  • Lymphotoxin-alpha / biosynthesis
  • Lymphotoxin-alpha / genetics
  • Lymphotoxin-alpha / metabolism
  • Membrane Glycoproteins / biosynthesis
  • Membrane Glycoproteins / genetics
  • Organ Specificity
  • Phenotype
  • Receptors, Lymphocyte Homing / biosynthesis*
  • Receptors, Lymphocyte Homing / genetics
  • Recombinant Fusion Proteins / pharmacology
  • Th1 Cells / drug effects*
  • Th1 Cells / metabolism
  • Th2 Cells / drug effects*
  • Th2 Cells / metabolism

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Neoplasm
  • CTAGE1 protein, human
  • Interleukin-13
  • Interleukin-5
  • Lymphokines
  • Lymphotoxin-alpha
  • Membrane Glycoproteins
  • Receptors, Lymphocyte Homing
  • Recombinant Fusion Proteins
  • L-Selectin
  • Interleukin-10
  • Interleukin-12
  • Interferon-beta