Cardioprotective effect of interleukin-10 in murine myocardial ischemia-reperfusion

Eur J Pharmacol. 1997 Sep 10;334(2-3):157-63. doi: 10.1016/s0014-2999(97)01149-7.


We investigated the cardioprotective effects of rat interleukin-10 in a murine model of myocardial ischemia-reperfusion (20 min ischemia, 24 h reperfusion). Interleukin-10 (100 microg/rat) administered 15 min prior to reperfusion, significantly (P < 0.01) attenuated myocardial injury compared to rats receiving only 0.9% saline as a vehicle, as indicated by a reduced loss of myocardial creatine kinase from the ischemic-reperfused myocardium. Cardiac myeloperoxidase activity was also significantly (P < 0.01) attenuated by interleukin-10 within the ischemic-reperfused region compared to vehicle treated rats. To further investigate the mechanism of interleukin-10 we observed the in vitro adherence of neutrophil to rat vascular endothelium. Interleukin-10 treatment significantly (P < 0.05) attenuated neutrophil adherence to rat superior mesenteric artery endothelium stimulated with interleukin-1beta. Thus, interleukin-10 demonstrated significant cardioprotective effects as evidenced by a decrease in myocardial creatine kinase loss as well as an inhibition of neutrophil accumulation within the myocardium. It appears as though interleukin-10 mediates its effects, at least in part, by inhibiting leukocyte-endothelial interactions.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Adhesion / drug effects
  • Creatine Kinase / metabolism
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / physiology
  • In Vitro Techniques
  • Interleukin-10 / pharmacology*
  • Mesenteric Artery, Superior / drug effects
  • Mesenteric Artery, Superior / physiology
  • Myocardial Reperfusion Injury / enzymology
  • Myocardial Reperfusion Injury / prevention & control*
  • Myocardium / enzymology
  • Neutrophils / drug effects
  • Neutrophils / enzymology
  • Neutrophils / physiology
  • Peroxidase / metabolism
  • Rats
  • Rats, Sprague-Dawley


  • Interleukin-10
  • Peroxidase
  • Creatine Kinase