Cloning and sequencing of the mouse cDNA encoding a phospholipid hydroperoxide glutathione peroxidase

Gene. 1997 Oct 1;198(1-2):245-9. doi: 10.1016/s0378-1119(97)00321-1.

Abstract

Phospholipid hydroperoxide glutathione peroxidase (PHGPx), a selenoprotein, reduces the hydroperoxides of phospholipid, cholesterol, and cholesteryl ester in biomembranes. In this study, a full-length cDNA clone encoding the PHGPx was isolated from mouse testes using a RACE (rapid amplification of cDNA ends) technique. According to sequence analysis, the cDNA encodes a polypeptide of 197 amino acids (aa) that initiates the translation at ATG(145-147) and contains an inframe TGA selenocysteine codon. It also has selenocysteine insertion sequences in the 3'-UTR that are involved in the insertion of selenocysteine at an opal codon. Moreover, the mouse PHGPx contains the active-site residues Gln108 and Trp163 that interact with selenocysteine, and the N-terminal 27-aa residues that may act as a potential mitochondrial targeting signal. According to the deduced aa analysis, mouse PHGPx shares a high level of aa identity with pig (93.4%), human (92.9%), and rat (98%) PHGPxs. However, the PHGPx mRNA particularly showed a high degree of expression in testis. This suggests that the PHGPx in testis may have more than just an antioxidant function.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Consensus Sequence
  • DNA, Complementary / genetics
  • Glutathione Peroxidase / genetics*
  • Humans
  • Male
  • Mice
  • Molecular Sequence Data
  • Phospholipid Hydroperoxide Glutathione Peroxidase
  • RNA, Messenger / genetics
  • Rats
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Swine
  • Testis / enzymology

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Phospholipid Hydroperoxide Glutathione Peroxidase
  • Glutathione Peroxidase

Associated data

  • GENBANK/D87896