PCTAIRE 2, a Cdc2-related serine/threonine kinase, is predominantly expressed in terminally differentiated neurons

Eur J Biochem. 1997 Oct 15;249(2):481-8. doi: 10.1111/j.1432-1033.1997.t01-1-00481.x.

Abstract

PCTAIRE are members of a subfamily of Cdc2-related kinases that have been shown to be preferentially expressed in post-mitotic cells. To examine the neural functions of PCTAIRE, rat cDNA clones encoding PCTAIRE 1, 2, and 3 were isolated, and their expression patterns in the brain were analyzed. Among the three rat PCTAIREs, only PCTAIRE 2 was found to be specifically expressed in the brain. Furthermore, its expression was transiently increased during brain development, peaking 7-15 days after birth. Within the brain, PCTAIRE 2 was concentrated in the neuronal layers of the hippocampus and olfactory bulb, which mostly consist of post-mitotic neurons. In an immunocytochemical experiment, immunoreactivity for PCTAIRE 2 was detected in the cell bodies and extended neurites of neurons, but not in astrocytes. The PCTAIRE 2 protein was recovered in the particulate fraction and resistant to solubilization with non-ionic detergent, suggesting that PCTAIRE 2 might be present as a component of a large protein complex. An immunoprecipitation assay revealed that the PCTAIRE 2 was associated with Ser/Thr-phosphorylating activity for histone H1, and that its activity depended on association with a regulatory partner that can be released under high-salt conditions. These findings suggest that PCTAIRE 2 is a Ser/Thr kinase that might play a unique role in terminally differentiated neurons.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / metabolism
  • Amino Acid Sequence
  • Animals
  • Brain / embryology
  • Brain / enzymology*
  • Brain / growth & development
  • Cell Cycle
  • Cell Differentiation
  • Cells, Cultured
  • Cloning, Molecular
  • Cyclin-Dependent Kinases*
  • Embryonic and Fetal Development
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Gene Library
  • Hippocampus / cytology
  • Hippocampus / enzymology
  • Humans
  • Hypothalamus / enzymology
  • Molecular Sequence Data
  • Neurons / cytology
  • Neurons / enzymology*
  • Olfactory Bulb / cytology
  • Olfactory Bulb / enzymology
  • Protein-Serine-Threonine Kinases / biosynthesis*
  • Protein-Serine-Threonine Kinases / chemistry
  • Rats
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription, Genetic

Substances

  • Recombinant Proteins
  • Protein-Serine-Threonine Kinases
  • Cyclin-Dependent Kinases
  • PCTAIRE-1 protein kinase
  • PCTAIRE-2 protein kinase
  • PCTAIRE-3 protein kinase