Microsatellite-based Cancer Detection Using Capillary Array Electrophoresis and Energy-Transfer Fluorescent Primers

Electrophoresis. 1997 Sep;18(10):1742-9. doi: 10.1002/elps.1150181007.

Abstract

The development of sensitive, rapid, and accurate methods and apparatus for high-throughput short tandem repeat (STR) analysis will be critical for the use of microsatellite alteration in cancer screening. Here we show that STR-based bladder cancer diagnosis can be performed using capillary array electrophoresis and two-color labeling with energy-transfer (ET) fluorescent primers. Rapid (< or = 35 min) separations are achieved on capillary arrays using replaceable separation matrices and the allelic ratios are quantitatively determined with a precision of +/- 10%. With this precision, a variation of 20% was considered diagnostically significant. These methods provide a significant improvement in the speed, ease, and precision of STR analyses compared to slab gel electrophoresis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • DNA, Neoplasm / analysis*
  • DNA, Satellite / analysis*
  • Electrophoresis, Capillary / methods*
  • Energy Transfer
  • Fluorescent Dyes
  • Humans
  • Microsatellite Repeats
  • Repetitive Sequences, Nucleic Acid*
  • Urinary Bladder Neoplasms / diagnosis*
  • Urinary Bladder Neoplasms / genetics

Substances

  • DNA, Neoplasm
  • DNA, Satellite
  • Fluorescent Dyes